OK think I am loosing the plot, did my check up on the colony tonight and halfway through the frames found a different queen, golden in colour and unmarked, did a double take and then another, impossible! I must have been a big torpedo shaped drone? Finished going through frames and didn't find the marked queen, perhaps she was dead and the workers had made another queen, impossible as there hadn't been any queen cells.
So thought I had better go back through the frames again, sure enough can across the big golden coloured bee again, a few slides later found the small dark marked queen.
Hmm, got to have got this all wrong, but I have noticed bees are becoming a mix of dark and golden bees! OK it's impossible will have another look next time, maybe get a second opinion.
Also thought I would dust bees with icing sugar which is a treatment for varroa, garden was full of ghostly coloured bees flying about:-) They didn't seem to mind too much but did look strange.
Actually they are pretty well behaved these days.